At Shire, we are proud and grateful to employ military veterans and thank them for their contributions to our company and our country. See where your military experience can play a role at Shire by using our military skills translator job search tool below:
Req #: 232937
Department: DEPARTMENT OF NEUROLOGY
Posting Date: 04/24/2024
Closing Info: Open Until Filled
Salary: $5,427 - $7,100 per month
Shift: First Shift
Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230701_a11y.pdf )
As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world.
UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.
DUTIES AND RESPONSIBILITIES The Research Scientist/Engineer 3 (E S UAW RSE) has the following responsibilities:
Mouse Work (40%)
Assisting in maintaining the mouse colony.
Sacrifice via intraperitoneal or subcutaneous injection of anesthetics and preparation of plasma samples.
Dissection of tissues, including micro-dissection of brain tissue into sub-regions.
Isolation of biomolecules (DNA, RNA and Protein) from tissue and cell samples.
Treatment of mice via intraperitoneal, intravenous and subcutaneous injections.
Injection of oligonucleotide drugs intracerebroventricularly.
Transcranial perfusion of mice with PBS and fixatives to generated fixed mouse tissues for histological analysis.Cell and Molecular Biology (40%)
Isolation of biomolecules (DNA, RNA and Protein) from tissue and cell samples.
Quantification of target gene expression with quantitative real time PCR (QRT-PCR) or related techniques.
Quantification of target protein expression with western blots.
Staining of tissue slides for immunohistochemistry.
Analysis of immunohistochemical images using existing protocols in the ImageJ software package.
Significant experience with cloning plasmid DNA, including propagation of plasmids in E. coli, sub-cloning.
Generation and validation of guide RNAs for CRISPR/Cas9 experiments.
Sub-cloning guide-RNA and other cassettes into AAV-generation plasmids.
Gene expression using RNASequencing techniques.
Assays in immortalized cell culture including post-stressor viability, sub-cellular trafficking and enzyme activation assays.Analyzing Results and Writing Manuscripts (20%)
Reads and understands the literature around the topics of research.
Generates research outputs in the forms of slide decks, manuscript text and figures, progress reports to funding agencies, conference posters and presentations.
Careful record keeping using Microsoft Office and Cloud Based online Storage.
Statistical analysis of data using R.
Analysis of microscopy images using ImageJ and other programs. MINIMUM REQUIREMENTS
Bachelor's Degree in Engineering or Scientific Discipline
Four years of relevant experience Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration. ADDITIONAL REQUIREMENTS
Experience with microscopy (light, immunofluorescence and/or confocal).
Significant molecular biology experience.
Significant cell culture experience.
Significant experience with rodents in research, preferably in neuroscience research. DESIRED QUALIFICATIONS
Significant experience in analyzing genome-wide transcriptional datasets.
Experience with the R programming language.
Mouse Behavioral analyses.
Recovery surgeries in mice, including stereotaxic injections in rodent models.
Experience with primary mammalian cell cultures and/or iPSC cultures.
Experience with live-cell microscopy.
Graduate degree in related field. Application Process: The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.